spsI [2019-06-27 16:31:14]

glucose-1-phosphate thymidylyltransferase, spore coat polysaccharide synthesis

locus: BSU37840

pI: 5.39

mw: 27.62 kDa

protein length: 246 aa

gene length: 741 bp

function: rhamnose biosynthesis, spore coat polysaccharide synthesis

product: glucose-1-phosphate thymidylyltransferase

essential: no

ec: 2.7.7.24

synonyms: ipa-71d

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.7|Biosynthesis of rhamnose (for the exosporium)]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 3,885,239 3,885,979

The protein

Catalyzed reaction/ biological activity

• alpha-D-glucose-1-phosphate dTTP - dTDP-glucose [Pubmed|22960854]

Protein family

• glucose-1-phosphate thymidylyltransferase family (according to Swiss-Prot)

Structure

• [PDB|3HL3] (from B. anthracis, 55% identity)

[SW|Localization]

• outer spore coat [Pubmed|26577401]

Expression and Regulation

Operons

• genes: [gene|219B0AAFB4ABB7E97A906553B73423F712505E27|spsA]-[gene|9E577B935FAB9605DAF83353E907A6561F11CAC1|spsB]-[gene|38DBA751456A0C0EC5D028939ADB8860ADA28F94|spsC]-[gene|2648D9E356683F5E9C00C40F0A6BBD1933322B09|spsD]-[gene|F27921CD9B89EB16AA57ACECEDBF21ED383629F0|spsE]-[gene|222F7086A214A427174E188CB1FEE4A8B98D303C|spsF]-[gene|9B34C9DBEC5B84A631CDFBEE8DF44384FF5C03B0|spsG]-[gene|8A80A058F270C134891CBCE7EBD8D7A23797F599|spsI]-[gene|90D505CAA4FAAEB6A6AF5C278E70DF714FACB4DA|spsJ]-[gene|4A4C5E6C1DFD46BC3CF9AEBA3FC1577767FF8465|spsK]-[gene|F87238E9B22F967370971ABA4F911EFAC4F559B5|spsL]
  description: [Pubmed|9353933]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|26577401], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15383836], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|25239894,15383836], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

  regulation

  • expressed during [SW|sporulation] in the mother cell ([SW|SigE], [protein|search|SigK], [protein|search|GerE]) [Pubmed|26577401,25239894,15383836]

  additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• BKE37840 ([gene|8A80A058F270C134891CBCE7EBD8D7A23797F599|spsI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATTAACACTTCCTTACA, downstream forward: _UP4_CGGAAAGGACAGGACGAAAA
• BKK37840 ([gene|8A80A058F270C134891CBCE7EBD8D7A23797F599|spsI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATTAACACTTCCTTACA, downstream forward: _UP4_CGGAAAGGACAGGACGAAAA

References

• 9353933,15383836,12884008,21821766,22960854,25239894,26577401